The Scientific Quarterly

ANNOUNCING OUR PROFESSIONAL MOLECULAR BIOLOGY WORKSHOP
JULY 21 – JULY 25, 2008

By The Science Creative Quarterly

MOLECULAR BIOLOGY TECHNIQUES WORKSHOPS (SUMMER 2008 PROGRAM):

Now accepting registration
To register, please contact Dr. David Ng at db@interchange.ubc.ca

(1) ONE WEEK VERSION
Dates: July 21st – 25th, 2008
Price: CAN$1250 (does not include room or board)

Reviews and Testimonies
Can be found here.

INSTRUCTOR: Dr. David Ng
DESCRIPTION: This intense one week workshop will focus on a myriad of different techniques used in the molecular manipulation of DNA, RNA and protein. Primarily aimed at researchers who are new to the area, or familiar but require a quick updating. Procedures that will be covered both practically and theorectically can be seen in the table of contents of our lecture notes Note that there will be some modification to the content as we update our syllabus for the July session:

ONE WEEK WORKSHOP LECTURE NOTES (previous pdf lecture notes available):
(previous pdf for 1 week lab manual also available)

CONTENTS: (note that the order is due to experiments).

1.1 DNA: Your Friend.

2.1 GENOMIC DNA: What and Why.
2.2 GENOMIC DNA: Cell Lysis.
2.3 GENOMIC DNA: DNA Purification.
2.4 GENOMIC DNA: DNA Precipitation.

3.1 MORE ON DNA: Quantitation.
3.2 MORE ON DNA: AGAROSE GEL ELECTROPHORESIS.
3.3 MORE ON DNA: NUCLEIC ACID PURIFICATION KITS

4.1 Cloning: What is a Vector All About?
4.2 Cloning: Which Vector Should I Use? (Part 1)
4.3 Cloning: Which Vector Should I Use? (Part 2)

5.1 ENZYMES: Restriction Endonucleases (Part 1).
5.2 ENZYMES: Restriction Endonucleases (Part 2).
5.3 ENZYMES: Ligases and Phosphatases – The Jist.

6.1 Transformation/Transfection: Getting DNA into your host.

6.2 PLASMID PREPS.

7.1 PROTEINS: Your “High Maintenance” Friend.
7.2 PROTEINS: The Western Blot – Part 1.
7.3 PROTEINS: The Western Blot – Part 2.

8.1 RNA: Your “unreliable” friend.
8.2 RNA: Isolation and Purification.
8.3 RNA: Gene Expression.

9.1 POLYMERASE CHAIN REACTION – The Jist.
9.2 PCR – A brief tour of the reaction specifics.
9.3 PCR – A brief tour of troubleshooting options.
9.4 REAL TIME PCR.

APPENDIX A: Replication, replication, replication…
APPENDIX B: Hybridization and Stringency for Dummies.
APPENDIX C: A Selection from the Merck Manual.

LOCATION:
Located in the heart of the UBC campus, the Michael Smith Laboratories is a testament to the vision of its founding Director, Dr. Michael Smith. Under his leadership, a gifted team of young scientists were recruited. These scientists have gone on to develop internationally renowned programs of research and training. The second and third floors of the new building are dedicated to the research facilities of the former Biotechnology Laboratory. The Stewart and Marilyn Blusson Education Forum is located on the ground floor and is open to the public. The molecular techniques workshops are held in the teaching lab,room 105 of this forum.
(click here for detailed directions)

REGISTRATION DETAILS:
Registration is essentially through first: an email inquiry for space (db@interchange.ubc.ca), second: a verbal commitment and then third via payment. Your place is essentially secured with payment, which more or less equates to a first come first serve mechanism. This payment would be a CAN$1250 cheque (or equivalent) payable to “The University of British Columbia” and sent to

Dr. David Ng
Michael Smith Laboratories
301-2185 East Mall,
University of British Columbia
Vancouver, BC, CANADA V6T 1Z3

If you prefer us to send an invoice, please let us know and we can arrange that accordingly. Note that we can accommodate a maximum of 16 clients, but on occasion up to half of these spaces are already reserved for predetermined group clients. Therefore, it’s best to put your name down as soon as possible if you are interested in the workshop.

REFUND POLICY
Your spot in the workshop is secured when we receive your payment. The deadline for receipt of payments is 3 weeks prior to the workshop start date. Unfortunately, we are unable to issue any refunds after this deadline has passed.

DAY TO DAY SCHEDULING DETAILS:
Workshop will begin each day at 9am sharp and usually end around 5:30pm. A detailed final schedule and syllabus will be released to clients as the date draws nearer.

MATERIALS:
All paper materials will be provided on the first day of the workshop. Downloadable versions will be available a week before the workshop begins. Whilst we do not require the clients to “study” these documents, we do ask that clients take a moment to peruse the first day practical materials. Please also bring a lab coat – if one is not available, please let us know and we will make arrangements accordingly. All other safety gear is provided at the workshop.

ACCOMODATIONS:
Here are some accomodation options that are basically on campus. Costs involved would vary (I think the most budget option would be the Vancouver Youth Hostel which is about a 15minute bus ride away). The closest would be those of Gage through UBC conferences. The others (except for point grey house) are all a relatively short walk away.

International Youth Hostel at Jericho Beach
UBC accomodations (on campus – note there are only 47 available)
St. John’s College (on campus)
Green College (on campus)
St. Andrew’s Hall (summer only)
Point Grey House (off campus, but only 10 minute bus ride away)

Alternatively, Downtown Vancouver offers a variety of accomodation options, but would entail about a 30-40minute bus ride each way. Depends on your preference since the Campus is pretty quiet at night time, whereas other areas would be more lively. Go to www.expedia.ca, and select:

hotel > near an attraction/vancouver > type in “University of British Columbia”

Usually the out of town clients make use of a little extra time after or before the workshop in visiting some of the sights Vancouver has to offer. I often strongly recommend this since the city and surrounding locale are really quite spectacular. In particular Whistler Blackcomb is a world famous ski/outdoor resort, and is only a 2 hour drive away. Ski season usually opens in mid November (click here for more info)

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A PROCRASTINATOR’S GUIDE TO THE UNIVERSE (OR IN DEFENSE OF DELAYING THE INEVITABLE)

By Anna Meredith

Empirical research would suggest that almost 90% of people are chronic procrastinators, with acute exacerbations occurring most frequently in student populations[1]. Given the incidence of this condition in the general population, it appears possible – even likely – that this behavior confers selective advantage, and since this trait enjoys continued popularity and prevalence in our society, one could suggest – as I will – that there is an evolutionary basis to its existence. Furthermore, as a procrastinator first and scientist second, I feel I can speak to some of the overlooked benefits of this type of behavior.

To procrastinate is to “delay or postpone action” as defined by the Oxford English Dictionary, and is derived from the Latin procrastinare, meaning “to defer till the morning”. As expected, and true to its British roots, the OED provides us with a diplomatic, dispassionate definition. Across the Atlantic, the official dictionary entry takes on a slightly less impartial tone, with Merriam Webster offering this take on my favorite pastime: “to put off intentionally the doing of something that should be done”.

Procrastination gets a bad rap. A quick search on Wikipedia (the go-to site when beginning one’s research on any topic, and coincidentally, a treasure trove for the true procrastinator – more on this later…) reveals that procrastination is a coping mechanism for those anxious about starting or completing “any task or decision”[2]. And apparently, procrastinating chronically is indicative of an underlying psychological disorder. But this isn’t news to anyone who has ever intentionally put off something that needed to be done: everywhere we look there are books, seminars, motivational speakers and websites all eager to teach us how to be more effective, more decisive, more efficient. Procrastination is public enemy number one in our quest to do ever more in ever less time. Type “procrastination” into Google, or your search-engine of choice, and apart from the requisite Wikipedia entry, the vast majority of the 5,900,000 search results deal with how to overcome this calamitous complaint.

Perhaps because of some perverse desire to be contrary or possibly due to my ‘underlying psychological disorder’, I feel that procrastination can be beneficial in maximizing the utility of one’s time. Through the myriad benefits of procrastination, which I shall proceed to list below, people who procrastinate well can get more accomplished, more efficiently and with greater success than those who follow the tedious mantra of good time management. The following will outline the common features of procrastination to which most can relate, I hope to build a case to clear procrastination’s name and demonstrate how such delay tactics can enrich our lives and optimize our limited time.

PRESSURE = GOOD!

As we all know,

Let’s assume that area represents time till due date. With this we can see that for equal force (task to be done), as area decreases (time is ticking…) pressure will increase proportionally. Sure you could get started on that paper that’s due next month right now, but the pressure of the task will be so low as to be unlikely to power any sort of progress, certainly not efficient progress. But wait until the night before, and by Jove, the pressure build up will cause those words to come flying out of you!

GET ALL YOUR DUCKS IN A ROW

Procrastination is active avoidance of the task at hand, but thoughts about this task tend to diffuse passively into one’s brain during the procrastination process. It is an unfortunate occurrence and requires ‘active transport’ to remove all traces of task related thoughts from the mind – hence the sudden fascination one has with today’s episode of Entertainment Tonight when faced with a tax return to complete, or a data set to analyze… What is new with Britney? Is Angelina Jolie pregnant? Again? These are important questions, the significance of which grows exponentially as deadlines near. All the effort we expend ridding our minds of the jobs we avoid inevitably leads to much thought going into these very tasks. And the more one tries to avoid a task, the more one inadvertently thinks about it. These thoughts are not futile, but rather like tiny snowflakes accumulating to create an avalanche of productivity once the appropriate moment is reached. Bottom line: subconscious preparation gets the job done with minimal effort.

Every year I complete my tax return. I do this, without fail, on the evening of the last day for submission. Being as how I am a student, and therefore do not have any money, even Revenue Canada cannot figure out a way to claim I owe them taxes. So in reality I could opt not to file my return, knowing full well that these types of organizations only pursue those who owe them money and are quite laid back when the debt goes the other way. Nevertheless, as a responsible citizen, I fulfill my duty and fill out the requisite forms, send them to our great government and wait patiently for my meager refund cheque to arrive six months later. In my months of procrastination and avoidance, brief pangs of guilt compel me to assemble a pile of relevant correspondence, receipts and forms. I add to the pile every other week or so, with the general intention that perhaps that will be the day I file the return early. I secretly know that I would never do such a thing, but the delusion serves its purpose, and so on April 30th, when I have only an hour to complete my filing, I can find all the necessary paperwork and finish the task efficiently and on time.

DON’T WASTE TIME AND ENERGY DOING STUFF THAT MIGHT NOT NEED TO BE DONE

One of the greatest, and least appreciated, benefits of procrastination is the fact that if you put something off long enough you may not need to actually do it. Recently, whilst discussing the preponderance of terrible data and negative experimental results I was experiencing with fellow lab minions, we formed the brilliant idea that a journal should be created to accommodate such data. If there ever were a journal in which I could publish prolifically, this would be it. And given that most of us engaged in scientific research experience more failures than success, imagine the wealth of data that exists! We excitedly debated the possibility of such a magnificent publication, extolling the virtues of sharing with the world (or at the very least, geeks like ourselves) the fact that one cannot successfully detect this or that protein in such and such a cell line… If we were a group of go-getters, perhaps we would have done something about this, but thank goodness we procrastinated! Turns out those evil geniuses at Harvard beat us to it with the aptly, yet dully, named Journal of Negative Results in Biomedical Research[3]. Hmmm… our journal would have had a far snazzier title; International Journal of Garbage, Research in Futility or simply Crap [4]. This approach also works with taking out the recycling: waiting long enough may compel your roommate to complete the task, and the only cost related to this tremendous benefit, is occasionally walking around a pile of empty beer bottles. The key here is energy conservation. A convenient byproduct of this approach is also time conservation.

LITTLE THINGS COUNT

My car is never cleaner than when I have an important task to complete. It is thoroughly vacuumed, the rims are polished, even the glass has been windex-ed. My closet is also immaculate at these times. The correlation has not escaped my attention. In the long summer months, when days are long and there are many fun activities in which to partake, when school is out for the year and there are no assignments, when co-workers take vacation and things slow down just a little, my car and closet are disaster areas. There is simply no time to complete the menial tasks – nothing to focus attention on the details. Having an important deadline confers upon one myopic vision. Suddenly trivial every-day chores gain new meaning, and more importantly, they get done. And not just done – done well. The thoroughness of my cleaning routine is astounding in times of stress, for I couldn’t possibly read that paper when the kitchen is in disarray! Or prepare that presentation when my tires need to be rotated. How could I contemplate preparing my grant application when I haven’t written to Grandma since last Christmas? Procrastination can therefore offer some respite from a hectic lifestyle, and allow one to slow down and focus on the details – which although seemingly trivial, are the stuff of life, and deserve our attention.

TAKING THE LONG WAY HOME

It has been said that the greatest discoveries happen by accident. Daydreaming is a form of avoidance which can certainly be classified as procrastination, yet has lead to a great many advances, particularly in science. Einstein was prone to prolonged daydreams, and it was during one such reverie that he is said to have fine tuned his Theory of Relativity [5]. For some, procrastination results in action, whether organizing socks or cleaning closets. For others, procrastination results in inaction and a state of contemplation. Others still attempt their baneful task, but become easily distracted by tidbits of information they find along the way. Which brings us back to Wikipedia – nowhere else can one spend endless hours wandering through a maze of information, each article linked to another, and another even less relevant, but more fascinating, entry. Oh, the things I have learned by total fluke! The Sweetener Wars, the drama that was New Coke, the fact that tomatoes are berries… and what is a pumpkin you may ask, well, thanks to an evening of procrastination, I can tell you that it is ALSO a berry. Mind-blowing.

In order to get a good evolutionary perspective on my hypothesis I decided that it might be a helpful to contact an evolutionary biologist. Unfortunately, due to time constraints (resulting in part from ‘delaying the inevitable’ ) I was unable to locate one in time. I do however have a friend who counts himself part of the Creationist camp, and I briefly considered ‘taking the inverse’ of his ideas on this question as a ballpark gauge of what my elusive evolutionist would have said . Unfortunately the conversation didn’t get far past the “evolution” stage, and consisted of my being informed that God gave us free will and that sloth is a mortal sin. All good points, but not really helpful for my purpose. So there goes my attempt at being fair and balanced [6]. I shall have to draw my conclusions alone.
The rewards of procrastination are plentiful, and those listed above are merely a sampling of some of the ways delaying the inevitable can be of benefit. Each one of us can add more elements to said list, reflecting the diverse and innovative ways humanity has evolved to deal with onerous tasks. The features of procrastination are tailored to optimize the efficient utilization of time and energy, while providing sufficient time for intellectual diversion and doing so can only serve to advantage those who practice these tactics. I have already admitted my chronic patterns of procrastination and in doing some research on the topic have been able to appreciate the prevalence of this behavior amongst my friends and acquaintances. It appears that people instinctively know the minimal time and effort they need to expend to complete a given task satisfactorily. Procrastinating is merely a rearrangement of priorities, and the negative connotations associated with these delay tactics are manufactured by societal beliefs. If anything needs to be rectified it is the guilt associated with procrastination, not the act of deferment itself. So I say to my fellow postponers – be proud, be productive, be a procrastinator!

FOOTNOTES
1. By empirical research, I mean that I looked at myself, my colleagues at work, and fellow students at school, and decided that we constitute a representative sample of most of humanity. It is possible that I am surrounded by a bunch of slackers and that these observations cannot be extrapolated to the population at large.

2. http://en.wikipedia.org/wiki/Procrastination

3. http://www.jnrbm.com/

4. My favorite journal name is Shock. Our Crap journal would seek to emulate the genius of this title.

5. http://www.smithsonianmag.com/science-nature/einstein

6. Whilst procrastinating momentarily during the writing of this paragraph I counted the number of times I used the word “procrasti-“ [22 times thus far] and have decided to mix things up a little by varying my terminology.

7. I have formed a hypothetical equation, which looks something like this:

It’s a work in progress…

8. Although I may have achieved this in the Fox News sense at least…

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Originally from London, England, Anna now calls Vancouver home, and is comforted by the similar gray and rainy weather these cities enjoy year-round. Anna enjoys drinking wine and singing along to foreign songs in languages she cannot understand. She daydreams of moving to Spain, opening a tapas bar and becoming a flamenco dancer. When not daydreaming, Anna works on her graduate degree in cardiovascular pathology, happy in the knowledge that wine is good for the heart.

INNUMERACY

By Katelyn Sack

White pear blossoms blurring on the downhill stride;
cloud veins branching, bursting blue-blood lights and streaks;
marmalade fractal-smudged bites in a Sunday;
points on the mounts’ horizon, range upon range
dipping over the edge of the world;
angles drawn with straight lines on that world,
bending Euclid, because they can,
because they must – no limit to truth when the paper
crumples, crumbs of calculations dropping off like forsythia-yellow stars.

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Katelyn Sack is a writer, painter, musician, nanny, medical botany researcher, and political economist residing in Charlottesville. Her recent work has appeared in the UK Guardian, McSweeney's Internet Tendency, The Science Creative Quarterly, Yankee Pot Roast, and Opium Magazine online.

THE HMS BEAGLE PROJECT – A SCIENCE CREATIVE QUARTERLY PIN UP (NO. 3)

By Peter McGrath and Diana Sudyka

(CLICK HERE FOR PIN-UP POSTER – pdf file ~200k)
– We suggest photocopying at 129% – LTR to 11×17 –

My wish was to be buried in the Churchyard at Downe. Now I find Mr Huxley, thumbing his nose at the Queen for refusing me a knighthood, arranged to have me planted in that mausoleum Westminster Abbey. He knows I hated London. And burying an agnostic in such a place is carrying whimsy just a little too far.

So I was delighted when a mob of angry biologists and historians broke into the Abbey one night and removed my remains with a view for a quiet burial in the village I loved. But no. Someone called Dawkins had a better idea. I don’t remember corresponding with him. Maybe a friend of that ghastly man Marx who kept sending me those books. Did he come to anything?

Anyway, I woke up in a thunderstorm, a bolt in either side of my neck, Dawkins on the phone saying, ‘there’s another book in this!’ My re-emergence into society in my bicentenary year has caused something of a stir, not least to myself.

Those of you who have actually read my writings (as in not just provide opinion on them) will know that I am a retiring man, not much given to attending either science or society functions. But I could not resist an invitation to visit my old friend, the Beagle. As I wrote not long before my first death, my time aboard her was the most important event in my life: without her I would have become a clergyman.

So we took the train down to the coast and there she was. A brand new HMS Beagle, sitting alongside trim and pretty as though she had just been launched in 1820. My heart gave a lurch (which worries you, when you have died) when I saw her again. The decks were clustered awaiting my arrival, and the welcome I received was embarrassing.

Still. the moment I stepped aboard, I felt the clammy sweat, the salivation of my old nemesis: seasickness. I suffered it for five years and frequently wished myself dead. Some observant soul saw this: ‘I know how you feel. Every time we go out I’d throw me ring up if I didn’t take these.’ He handed me two tablets. ‘Or you could always eat some jam.’

‘Do preserves cure seasickness?’ I asked, amazed. ‘No, it just tastes nicer when it comes up.’ I shall have to catch up with the new humour, I see. But it was good to be made game of: I am a bearded man who has been resurrected from the dead, but I am no God.

The upper deck was much as I remember leaving in 1836: the wheel with Nelson’s immemorial words: ‘England expects every man will do his duty’. The Captain – he did not have Fitzroy’s haughty bearing – saw my affectionate glance at the helm and in an exasperated way said, ‘If that was today, Nelson would have to say “England expects every man will fill in a risk assessment.” Anyhow, look at this.’ The inanimate piece of glass sprang into colourful life. ‘GPS.’ I raised an eyebrow. ‘Global positioning system. It uses satellites 22,000 miles above us to calculate our position to the nearest ten feet. It has charts for the whole world.’

‘No more sextants?’ I asked. The daily taking of sights at noon and of shooting the altitude of starts was a great ritual every day aboard the old Beagle. ‘Oh, I still have mine, and my requisite tables. Sometimes, the electrics pack up.’

‘So the whole of the world has been charted? And they are stored in this small grey box?’

‘Yes. And watch this!’ He punched a button. ‘Depth!’

‘So no more casting weighted lines?’

‘No, Mr D. And here.’ The display changed again. ‘Radar. It paints a picture of the land and sea around us. We can see boats before they appear on the horizon, can sail safely through fog and storms.’ It looked like paintings my little Frankie used to do after Emma had been a little too free with the alcoholic tincture of laudanum. She used to give that a lot for colds. With so much changed, I suppose science will have found cures for such simple ailments by now.

My old stern cabin was very different to the one I shared with Stokes and King. Cramped still, but with cabins and…what did I feel? The captain had the shame to shuffle his feet. ‘Heating,’ he mumbled. ‘Air conditioning, too, when it gets too hot.’ A click and an electric bulb glowed
into life. I had seen these in a house, but on a boat. ‘And that?’

‘You haven’t heard of iPods, then?’

‘Something to do with plant reproduction?’

The captain shook his head and vanishing down a set of ladders told me to ‘mind my head’. My 200 year old knees cracked a bit. Age had stooped my frame, but still in the bowels of the ship, I had to hunch, and what a different sight to the stinking, rodent infested hole I had last seen in 1836.

‘Engines. 440 horsepower, folding propellers, push us along at eight knots when there’s no wind. And generators provide mains electricity for all you scientific types – the stuff you lot need – and 24 volts for the boat.’

Fitzroy sailed us around the world and I wrote The Origin of Species without electricity, I mused. We crouched as we walked between rows of gleaming boxes and hoses. ‘Diesel and water tanks. Freezers. These are for food, these are for your metagenomics samples.’

‘Metagenomics?’

‘Don’t tell him about that! It’ll kill him again!’ A voice yelled from behind us. ‘We’ve got to get him up to speed with the peas first….’

Moving past the captain, I confronted the hovering woman in the white lab coat. ‘What’s this about peas?’ I asked. And so, standing in the hold of HMS Beagle I was given a brief introduction to the science of genetics. Well, well. It seems monks are good for something after all – transmitting variation from one generation to the next. Not that monks do that, I mean.

Anxious not to delay my host, I emerged again into the main deck, now lined with small comfortable cabins, very different to the hammocks of my day. In between the cabins, a table was bolted to the floor. ‘Lab space. We’re doing what you did, Mr D. we’re going round the world, sampling the seas, looking at how the world’s changed since you saw it, how the rainforests have been cut down, the glaciers have melted, where the species have moved to, how many have gone extinct. A lot of the people here are doing it because of you. A lot of ‘em read the Voyage, the Origin, and that’s why they’re here.’

I felt humble that my work on this little ship had touched so many. And a pang of envy. Their breathable waterproofs. Heated cabins. Running water. Hot running water. Next I will learn that they will not have to wait to arrive at shore to communicate with the rest of the world. Maybe they are even able to identify specimens without consulting a museum or book!

The captain excused himself. ‘We need to be off, we’re taking a copy of your first edition of the Origin across the Atlantic to Harvard.’ He slapped the mainmast. ‘Give her a good try out before we go off round the world. Been a pleasure to have you aboard. Bit of a headwrecker for me, mind. I hope,’ he shuddered, ‘they don’t dig up Fitzroy and do the Frankenstein on him, bring him aboard. God, could you imagine that? Having the old skipper aboard too? But you know you’re welcome aboard. Anytime.’

The crew shook my hand one after another in a most civil manner and the captain saw me safely over the gangplank, and promptly started bellowing – as captains will – about starting engines, getting ready to slip. As my feet hit dry land, I was met by an attractive young woman, a species I have always liked. ‘Hi,’ she drawled, took my arm and walked me towards a car. ‘Yah. I’m Jocasta your agent for media-facing events. Oh, God look at you, we’re going to have to do something about that beard. We’ll have to book you in for a makeover and get your colours done, I’m thinking autumnal: you’re doing the all the TV breakfast shows tomorrow morning. Darling, you’re going to be huge. But Charles Darwin. A bit old, Victorian. Our branding people have been brainstorming and felt Chaz D…’

I tore myself from her grasp, opened my hand , gulped down the two seasickess pill I had been given and with a sprightliness surprising in a 200 year old, cantered up the gangplank. ‘You did say any time,’ I reminded the captain, ‘my science is a little rusty, but I think I can be of some use.’

- – -

The HMS Beagle Project aims to launch a replica of the HMS Beagle, an icon of scientific progress, for the bicentenary of Charles Darwin’s birth in 2009. She will circumnavigate the globe in Darwin’s wake, crewed by aspiring scientists and researchers. They will carry out original research both at sea and on land, updating Darwin’s observations, breaking new scientific ground and relating the adventure of science to enthuse a new generation of young students. If you support this vision please visit www.thebeagleproject.com for details on how you can help.

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PETER MCGRATH is an author who studied zoology at Liverpool University where he was introduced to ‘The History of the Idea of Evolution’ by Professor A.J.Cain. This sparked a lifelong interest in Charles Darwin, which when mixed with his subsequently developed love of sailing, led to the following terrible effect: Peter decided that Charles Darwin’s bicentenary in 2009 must be celebrated by building a sailing replica of HMS Beagle. To discuss flinging a sack of money into the HMS Beagle Project, helping a replica HMS Beagle sail the world in Darwin’s wake with young scientists aboard, please check out the project's website. - DIANA SUDYKA is a Chicago illustrator and artist. She has a Bachelors of Fine Art from University of Illinois is Urbana-Champaign, and a Masters of Fine Art from Northwestern University, Evanston, IL. She creates work for everything from book covers, album artwork, screen-printed rock posters, to watercolors for her avian blog, The Tiny Aviary, documenting her volunteer work for the Bird Division at the Chicago Field Museum of Natural History. She has always had a great interest in ornithology and natural history, and the work she produces is informed by that passion. To see more of her work, please click here.

JOURNAL CLUB FIND: SOMETHING ABOUT MATING WITH YOUR COUSINS IS A GOOD THING?

By The Science Creative Quarterly

An Association Between the Kinship and Fertility of Human Couples (pdf). Science 8 February 2008: Vol. 319. no. 5864, pp. 813 – 816

To quote… “greatest reproductive success observed for couples related at the level of third and fourth cousins” and as if tables like below really take away from the “ick” factor.

ABSTRACT:
Previous studies have reported that related human couples tend to produce more children than unrelated couples but have been unable to determine whether this difference is biological or stems from socioeconomic variables. Our results, drawn from all known couples of the Icelandic population born between 1800 and 1965, show a significant positive association between kinship and fertility, with the greatest reproductive success observed for couples related at the level of third and fourth cousins. Owing to the relative socioeconomic homogeneity of Icelanders, and the observation of highly significant differences in the fertility of couples separated by very fine intervals of kinship, we conclude that this association is likely to have a biological basis.

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IT’S OFFICIAL – INTRODUCING THE “SCIENCE CREATIVE LITERACY SYMPOSIA”

By David Ng

magnet.gif

Presumably, art and sciences interact a little like this?

The Science Creative Literacy Symposia is a new fieldtrip program offered at the University of British Columbia, and is designed to provide an engaging outreach experience for students at the Grade 6/7 level. Here, the intent is to combine elements of science exploration with expository creative writing with the aim of fostering skills in written literacy, scientific literacy, as well as develop appreciation in interdisciplinary connections.

- – -

Hosted by the Advanced Molecular Biology Lab at the Michael Smith Laboratories, and by the fine folks at the UBC Creative Writing Program, the fieldtrips will be held at the Michael Smith Building. Both research laboratory settings as well as spaces conducive for reflective writing activities will be used.

Each session involves a class of Grade6/7 students (about 30 max), and will encompass a timeframe from 10am to about 2pm: this includes a lunch break of 30 to 45minutes somewhere in the middle. Basically, the session will be run by two instructors; one a Graduate Student within a scientific discipline, and the other a Masters of Fine Arts student within the Creative Writing program. Each session will be designed to allow the elementary students to play the both the role of the scientist (perform a science experiment) and the writer (engage in a reflective writing exercise), with a concerted effort to provide linkage between the two skill sets. As well, it is hoped that there will be an outlet for publication of works from these sessions, whilst still allowing copyright to remain with the student. The fieldtrip is free (first come first served in terms of bookings), although transportation and lunch is not provided.

For more information about registration, participants, and fieldtrip content, please check out the Symposia’s mainpage.

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David is Director of the Advanced Molecular Biology Laboratory, the educational arm of the Michael Smith Labs. He's also the dude that edits the SCQ

A LITTLE HIKE

By Vania Chan

FADE IN:

Dialogue in Mandarin with English subtitles. An impoverished rural village in contemporary China.

EXT. A SMALL WOODEN HOUSE – - DAY

MAMA, a single mother of three children in her mid 30’s, walks along a dusty dirt road into a meek wooden house lugging a heavy pail of water.

A shabby cloth hangs in the doorway in place of a door.

INT. MAMA’S HOUSE – - DAY

A one-roomed house furbished with a couple of fold-out chairs and a few pieces of old wooden furniture.

One large wooden bed is in the far corner.

WEI, Mama’s youngest son aged 5, is lying in the bed being watched over by his GRANDMOTHER, a fragile little woman of 70.

Mama hovers over a small CRACKLING fire, cooking a small tin pot of rice over it. She fans the fire and looks over at Wei.

LU, and YUAN return to the house and walk towards Wei. Yuan places a wet towel over his face.

Lu is the eldest son, 11. He wears a faded undersized t-shirt with a corporate soft drink logo on it. Yuan, 9, is the only daughter.

INT. MAMA’S HOUSE – - DAY

AUNT LING, a nosy middle-aged neighbour, is conversing at a small fold-out table with Mama.

AUNT LING: You know, your health has been deteriorating ever since Jiang died. You can’t support this family by yourself. You can’t keep running to me for money.

MAMA I’m desperate…Wei is sick.

AUNT LING: You can’t pay me back.

Aunt Ling glances at Lu, who is teaching Yuan simple words by drawing the characters on the dirt floor.

AUNT LING (CONT’D): Lu looks pretty healthy and strong. You know, I heard a man is looking for healthy children to work for him. They pay pretty good. Up front too.

MAMA: …I don’t know…

AUNT LING: Look. You need money. He’ll give you the money and it’s a long-term contract. Just a little hike.

MAMA: He’s far too young. I can’t just ship him off to the unknown…

Lu watches the two women as they reason with each other. They are too engrossed in the conversation to notice.

INT. MAMA’S HOUSE – - DAY

Lu sits at the bed and watches Mama wash clothes in a plastic tub. He walks over and looks intently at her.

LU: Mama, I want to go work.

Mama, surprised, narrows her eyes at him.

MAMA: No. You’re not going. It’s too dangerous.

LU: I heard you and Aunt Ling talking. If I don’t go, how can we survive? I’m old enough to know I can help.

MAMA: I’ll find a way…you’re still a child, Lu. You don’t understand how complicated this is. There’s a lot of work involved. And you’ll be away for a very long time.

LU: Fang’s mother just sent him to work too. We haven’t heard from him yet, but I know it will be hard. I’m prepared to do this for our family. I can save Wei.

Mama looks at the floor. She slowly brings her gaze to Lu’s eyes. There are tears in her eyes. She hugs Lu and holds his head to her chest.

EXT. MAMA’S HOUSE – - DAY

The whole family stands outside the house. Lu takes a long look at his home. He turns and embraces his younger sister, brother and grandmother.

He is carrying a cloth bag stuffed with essentials.

They have an emotional farewell, and Mama guides Lu away from his siblings.

Their figures shrink as Yuan, Wei and the grandmother stand watching them as they disappear down the dirt road.

EXT. MOUNTAIN – - DAY

Mountainside. Dense forest. A steep rocky trail.

Mama and Lu trudge along a narrow pathway leading up the mountain, periodically dodging branches and roots growing onto the road.

The pair hike in silence. The branches SNAP under their weight.

They are surrounded by lush trees and wilderness.

EXT. MOUNTAIN – - DUSK

Mama slips — Lu lunges to her side to break her fall.

MAMA: Let’s take a break and have some food.

Lu takes a couple of stale buns from his cloth bag and hands one to Mama. They sit and take in their surroundings.

MAMA: Lu, you don’t have to do this…

LU: I told you Mama, I do. It’s my duty to help the family. I can’t sit back and watch this happen.

EXT. A CABIN – - DAY

Travel-worn, Mama and Lu arrive at a wooden cabin surrounded by thick brushwood. It is almost hidden.

MAMA: I think we’re here.

She knocks on the door.

A RUDE MAN, mid 30’s with a beard, flings open the door.

INT. CABIN – - DAY

Mama and Lu are sitting at a table talking to the man. Three other men are in a small bedroom to the right of them.

RUDE MAN: Two thousand Yuan for him. That’s a great deal.

MAMA: When can I visit him? What is he going to be doing? When does he get to come home? Where are you —

RUDE MAN: — don’t worry okay? Look, do you want the money or not? I don’t
have all day. Take it or leave it.

Mama faces Lu. He holds her gaze and nods reassuringly.

LU: I’ll see you soon okay Mama? Don’t worry about me. I love you.

She squeezes him tightly and kisses his forehead. They both fight back tears.

EXT. MOUNTAIN – - DAY

Mama stumbles down the mountain. Tears stain her face. She stops and looks fixedly at the money in her hand.

INT. CABIN – - DAY

Mama re-enters the cabin. No one is in the room.

She peers into the bedroom on the right. The door is half shut.

She sees Lu’s cloth bag near the door and moves closer to the room.

INT. CABIN BEDROOM – - DAY

Lu is lying on a wooden table. The rude man stands with his back against the door, leaning over Lu. Blood is DRIPPING from the table into a puddle on the floor.

Mama GASPS.

The man, startled, turns and reveals a motionless Lu. Lu lies with an open wound in his abdomen beside a bucket of ice.

Mama’s eyes grow wide and streams of tears fall down her face. She drops the money and starts to WAIL uncontrollably, running towards the room.

Men run out of the room with a BANG and hold her down. She kicks and YELLS and SOBS, bites a man, and manages to free herself.

She runs to her son. The rude man lunges after her with his scalpel. Mama SCREAMS.

CUT TO BLACK:

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Vania Chan is currently an undergraduate student at the University of British Columbia. She is interested in culture, especially film and music, as well as social issues and the way in which these two elements relate to one another.

THE MYTHOLOGY – AND POTENTIAL – OF THE PUBLIC INTELLECTUAL. LEARNING FROM RACHEL CARSON

By Kate Neville

“It is the public that is being asked to assume the risks…the public must decide whether it wishes to continue on the present road, and it can only do so when in full possession of the facts…”[1]

In her crusade to galvanize the American public against what she considered an insidious enemy masquerading as progress, Rachel Carson combined rigorous science and compelling prose to convey her message. Her book Silent Spring, published in 1962, sparked public interest in, and outcry over, the growing use of chemical products for controlling plants and insects. Her work is considered a key catalyst of the second wave of environmentalism in the United States[2], and has had a profound and lasting effect.

What is remarkable about Carson’s approach is not just that she used science to support her claims, but that she successfully combined complex scientific findings with a public action message in a campaign that swept across a predominantly non-scientific audience. Chapter by chapter, via the New Yorker – a literary magazine – she released a call to arms against the rampant use of pesticides. She countered the chemical industry’s metaphors of societal protection and advancement that justified their work, by adopting their imagery and using the language of nuclear threat and reckless destruction. She challenged her audience to become aware of the concerns and to understand the issues. She emphasized individual impacts, and focused on making her arguments straightforward and comprehensible, but did so without oversimplifying the message; Carson took her readers seriously and treated them as capable of making reasoned and responsible choices.

The persona of Rachel Carson has taken on archetypal significance within the modern environmental movement. Carson is seen as a pivotal character in the process of raising social awareness about the environment, and represents the power of individual action in changing public attitudes and policy directions. Her legacy in the environmental movement was the product of her success as a public intellectual, and she successfully tapped into powerful imagery to convey her message to a skeptical public. Carson’s message overcame its opposition because of her ability to frame her arguments in the language of its critics combined with her capacity to capture accessible and personalized stories.

One lesson to be learned from Carson’s work is the power of the integration of information and narrative – our science has value only to the extent that we use it wisely, while our policies have merit only when we understand the nature of the world to which they apply. Carson’s work is an illustrative case of the critical importance of interdisciplinary thought and communication across sectors, and is particularly valuable as an example to those of us who wish to effect global change.

Public Intellectuals and Activism

Public intellectuals are symbolic figures in society, who represent social ideas and utopic visions rather than individual perspectives. Their influence and power comes from their ability to channel public fears and desires in specific directions, and to portray problems in personally affective ways. Rachel Carson made a shift from scientist to public intellectual through her writings, particularly with the release of Silent Spring. She created a powerful and lasting legacy by awakening the public imagination to the potential threats of government-approved chemicals and sanctioned pesticide programs.

Carson did this by appealing not only to intellect, but also to emotion. She said:“[I]f facts are the seeds that later produce knowledge and wisdom, then the emotions and the impressions of the senses are the fertile soil in which the seeds must grow.” For the uninitiated, Carson transformed scientific inquiry into something fascinating, exciting, and mysterious, but not incomprehensible, nor unreachable.

Fuller invokes powerful mythological images by comparing the role of the public intellectual to that of Eris, in Greek mythology, who “who provided the prized apple that occasioned Paris’s judgment of the most beautiful Greek goddess, thereby unwittingly sparking the Trojan War”[3]. He states that “[i]n a phrase, the public intellectual is a professional crisis-monger,”[4] a label that highlights their role as one of social provocation and disturbance. Carson fulfilled this role with her uncommon attack on an accepted and comfortable paradigm. However, Alcoff warns that “the purpose of reason, after all, is to establish belief and justify claims of certainty, not simply to destabilize”[5]. Challenging accepted norms and beliefs is an important element of the role of a scholar and researcher in the public domain, but as there is also a productive element to the role of the public intellectual. Carson’s attacks moved beyond an exposition of the problems to prescriptive advice. She offered constructive advice to her audience, by suggesting avenues for action.

Multiple possible roles have been suggested for the public intellectual, including acting “as independent harbingers of moral truths, as ‘priests’ guiding and pricking the nation’s conscience, and as ‘prophets’ bearing witness to the sins of the nation and pointing the road to redemption”[6]. This suggests that there is a prophetic role for the individual, and takes the academic out of the sphere of positive research and into the realm of normative prescriptions and judgments. Cushman similarly talks about the “civic duties of intellectuals”[7] to not just popularize academic work by translating its jargon, but to actually influence public action. We may shy away from the messianic role alluded to in these accounts, but should not consequently abandon the project of reaching out beyond our disciplines and institutions. This is a role not widely accepted by academics within the current establishment – but it is to our detriment, as academics and as citizens, to ignore this public role.

There is, in our society, a hunger for connection, a readiness of so many people to be helped in reconnecting to something that has been distanced. Intuitive curiosity is not an elite sentiment; although the research we undertake may require specialized skills, knowledge, and intellect, the product of this work should not remain cloistered. As scientist and as thinkers, we share a moral imperative to communicate this knowledge, with each other, across disciplinary boundaries, and outside the academic and research spheres. This is true not only of the natural sciences, but also of the social sciences and humanities – we must strive to explain the phenomena of the world into which we gain insight, as they have an impact on the unfolding of our existence on the planet.

The Power of Rhetoric

Carson’s success was based in part on her ability to communicate in the language of the dominant scientific paradigm. As someone trained in the sciences, she had more credibility in her contestation of the science of chemicals than an activist from outside the discipline. Her work, as noted earlier, reflected meticulous research and careful documentation, and she excluded even potentially powerful images if she lacked adequate scientific substantiation[8]. Moreover, her claims were based on human health and safety from a biological perspective, rather than outlining only a moral objection to chemical use.

However, the power of her message came equally from her ability to capture the public imagination as from her carefully documented research. De Neufville and Barton wrote of the power of myth – which they defined as “stories which draw on tradition and taken for granted knowledge”[9] – in defining the understanding of policy problems. Interpretive narratives influence the approach taken to dealing with social issues, and shape the perception of both the problems and the proposed solutions. Carson understood the importance of creating convincing stories and images to convey a new interpretation of agricultural practices and chemical products. In the face of emotionally-laden language used by the dominant powers in the chemical industry and mainstream politics, that labeled some insects as “pests” and plants as “weeds,” Carson was able to tap into an alternate set of images of nature and weave her own narrative of a threatened utopia. She provided a rationale for questioning the chemical approach to the natural world that revolved around symbols of pastoral beauty, and re-shaped pests into a myriad set of insects, and weeds into plant communities with distinct ecological roles. The power of language in the dominant paradigms of academia and of the policy world underscores the importance of providing alternate metaphors and imagery for issues of public concern.

The Danger – and Necessity – of Standing Up

In a discussion of the social role of religious scholars, McCutcheon clarifies that a public intellectual is a “publicly accountable intellectual”[10]. This distinction changes the role of the academic within society, from an impartial observer to an engaged participant, by holding them responsible for their claims and analyses. Carson’s convictions in the validity of her claims and message – both factually and morally – were particularly important, because she was personally held accountable for their content and implications.

Carson was attacked as a person by the chemical industry she threatened – to discredit her message, they had to discredit her person. Carson was forced to defend not only her academic credibility but also her personal life. This criticism of both her person and her work illustrates the danger in provoking social reaction and in challenging status quo systems. It also highlights the necessity of having public figures who are willing to bear the burden of public scrutiny, and the importance of supporting the individuals who do this. While we may debate their arguments and the content of their claims (we need not agree with the message put forth by these public figures), we must nevertheless aim to support their role in society, and promote the dialogue that they engender.

For the position of a public intellectual to have transformatory power, an individual must transcend being a person with a message and must become an emblem of that message; however, the humanity of the individuals who take on these roles must be supported and protected. Rachel Carson’s lasting impression on the social environmental consciousness resulted from her ability to utilize meaningful symbols at a human scale to impart her criticism of mainstream social and political choices. Carson took on the burden of accountability for an unpopular message within the framework of scientific and societal norms of the time, and her legacy is in the bravery of that action.

We are, at the moment, at the edge of tremendous changes to our world. We are watching glaciers melt, seeing geological processes at tipping points, observing the effects of processes that we do not yet understand. We are also in a moment of political and social opportunity and upheaval, where the possibilities are open, and the direction has not yet been set. Our knowledge is piecemeal, incomplete, and uncertain; we are testing the limits of our understanding of our planet, and it is a time of great potential and grave danger.

We are poised at a unique moment in history that we cannot afford to let pass us by. In the academic world, we are in a position to forge the new directions, as we have incredible communities upon which to draw – we are connected through mechanisms of communication that allow collaboration and innovation beyond what we could accomplish individually. We should take from Rachel Carson the hope that her actions conveyed: that great change can come through research, that people do want to know more, and that narrative can bridge the gap. We need not all take on a public role to engage in this process: Carson’s influence came from her ability to synthesize work across many fields, which relied on the willingness of many researchers to take the time and effort to share their findings with her, and explain the significance and the debates. We must support our public intellectuals – question their conclusions, but champion their causes; critique their claims, but provide them with alternative information. We need to communicate our research more clearly, participate in dialogue and explanation, and engage with the issues of our time in collaborative, constructive, critical, and public ways. We have the potential to effect great change, even in the most improbable of cases, and even on the most intractable of problems.

References

1. P. 13 in: Carson, R. 1962. Silent Spring. Houghton Mifflin Company: New York.

2. Scott, W.R. 2002. Organizations and the Natural Environment: Evolving Models. Chapter 20 in: Organizations, Policy, and the Natural Environment: Institutional and Strategic Perspectives. Eds. Hoffman, A., and M. Ventresca. Stanford University Press: Stanford, CA. Pp. 453-464.

3. P. 148 in: Fuller, S. 2006. The Public Intellectual as Agent of Justice: In Search of a Regime. Philosophy and Rhetoric. 39(2): 147-156.

4. Ibid.

5. P. 533 in: Alcoff, L.M. 2002. Does the Public Intellectual Have Intellectual Integrity? Metaphilosophy. 33(5): 521-534.

6. P. 262 in: English, R. and M. Kenny. 2001. Public Intellectuals and the Question of British Decline. British Journal of Politics and International Relations. 3(3): 259-283

7. P. 330 in: Cushman, E. 1999. The Public Intellectual, Service Learning, and Activist Research. College English. 61(3): 328-336.

8. Carson’s comments about an eight-legged frog in her drafts of Silent Spring (accessed at the Beinecke Library at Yale University), noted that she would include the information if she could find convincing evidence. Its absence in the final version of the book suggests that she was not able to substantiate the claim.

9. P. 181 in: De Neufville, J.I., and S.E. Barton. 1987. Myths and the definition of policy problems: An exploration of home ownership and public-private partnerships. Policy Sciences. 20: 181-206.

10. P. 453 in: McCutcheon, R.T. 1997. A Default of Critical Intelligence? The Scholar of Religion as Public Intellectual. Journal of the American Academy of Religion. 65(2): 443-468

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Kate started a PhD in political science at UBC this year, looking at conflict and cooperation over freshwater, particularly for rivers that cross international borders. When not immersed in coursework, or reading about dams and drought, she can usually be found outdoors.

OBSERVATIONS / REFLECTIONS ON STATUS QUO

By Joyce Wong

1. Trees in the city (at winter)

we are surrounded by pavement.
shallow roots and
bare branches.
perhaps strung with christmas lights
or strangled by these strings of lights?
fragile / frail / awkward
we stand in parking lots, by sidewalks
– illusions that the city has not been
taken over by concrete and pavement.
birds avoid us
as if we don’t belong.

2. Vending machines… and their opposites

picture, for a moment,
a vending machine.
what does it see?
when we participate in
this exchange –
money for goods, or not-so-goods.
material profit, material waste.
do you think we sometimes act like
buying machines?

3. Graffiti

dull surfaces defaced
by thoughtless scribbles
of rebel expression.
bold eyesore.
letters entangled,
hardly readable.

but there is a deeper wound,
a scar that we leave / inflict
upon this planet / a betrayal.
sometimes without thinking
or noticing / careless,
without regard (not watching
out for, not guarding) –
sometimes deliberate.
we are the vandals, the culprits,
the perpetrators who perpetuate
this crime, this graffiti.

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Joyce is a second year Arts student who may be found observing and pondering, and sometimes writing. She enjoys the language and music of creativity, as well as many of its colours and flavours, including the bittersweet taste of art that raises awareness of our human fallaciousness.

TARGETING TELOMERES AND CANCER FOR ‘DUMMIӘS’

By Sandy Henderson

For ‘Dummies’ is a simple yet powerful publishing genre to alleviate the anxiety and frustration people feel about understanding complicated material by mocking them with insightful and educational descriptions, making difficult material interesting and easy to understand. One of the most widespread and complicated diseases affecting us today is cancer. Everyone’s heard about cancer, but how bad is it really? Super-duper bad. Canadian statistics alone state 4 in 10 Canadians will get cancer in their lifetime. That number is getting so high; almost everyone is going to know someone affected by cancer. This reality should be enough that everyone ought to know about this disease—sadly this is not the case.

The Diversity of Cancer

Cancer is the name given to a huge group of diseases of which there are over 200 different types. Cancer can attack any organ and/or any tissue. Cancer can affect people of all ages from infants to adults. All cancer diseases are marked by aggressive cells, with similar properties. They can grow and divide without any constraints, they can invade and destroy adjacent tissues, and in some cases they can even spread away from the location of origin. But what really is cancer? Cancer isn’t something you can ‘catch’. At its most fundamental level cancer is one or more cells gone wrong. It starts as a single cell in your body with a mutation in its DNA, a mutation in the ‘instruction’ manual of the cell. The mutation changes your otherwise normal cell into an aggressive cancer cell. With each different cancer type there are one or more mutations related to that diseased state. Different mutations lead to different disease outcomes. Different mutations affect different tissues. Most importantly, different mutations lead to different therapies. Without even understanding the molecular mechanisms behind cancer, the diversity of cancer is overwhelming.

Given the diversity of cancer, one might imagine the fight against cancer and the development of drug targets to cancer would be as equally diverse. It is. Just in terms of treatments there are a huge quantity options. Most doctors today rely on their mammoth arsenal of treatments to inundate cancer. Everything from surgery, radiation, chemotherapy, and/or hormone therapy is thrown at cancer in the clinic. Most of these treatments are used in combination and in particular cases. However, none are particularly curative. What targeted cancer therapy needs is a link between most, if not all, cancers. There is hope and it’s called the telomere.

Telomeres and Becoming Immortal

The history of science is filled with mistakes–usually based on poor assumptions, which require ingenuity and imagination to overcome. The field of cancer is no exception. In very early work (before the 1960’s) scientists believed cells isolated from organisms possessed the ability to proliferate indefinitely. Of course, there were signs suggesting otherwise, especially when a few scientists noticed that cells given to them from colleagues seemed to stop proliferating after only a few weeks worth of growth. Whenever this problem was discussed, it was assumed that the growing conditions in that laboratory insufficiently represented the cell’s natural conditions, and therefore caused these cells to stop proliferating. It wasn’t until sometime in the 1960s, that Leonard Hayflick’s pioneering work challenged this assumption. Hayflick discovered that connective tissue cells (fibroblasts) isolated from an organism possessed only a limited proliferative ability and, in fact, these cells would only pass through a predetermined number of division cycles. No matter what he did, these cells stopped any further proliferation. These fibroblasts were not dead, but stuck in a state of stasis, no longer possessing the ability to divide. Hayflick’s work demonstrated that the previous assumption that all cells have an unlimited proliferative ability was wrong. In fact, Hayflick’s work lead to our current understanding of cells in which, cells possess an internal clock mechanism capable of counting the number of divisions a cell has made and stops any further divisions after a set number (Termed: The Hayflick limit).

So cells have an internal clock, what does this mean? Scientists quickly realized there was a link between the internal clock in cells, aging and cancer. The idea is, as we age our cells replicate, divide and eventually die. The older we get, the older our cells get. Infants have ‘young’ cells and adults have ‘older’ cells. This process arises through the normal wear and tear with age, termed biological aging. However, cancer cells are an exception. One of the hallmarks of cancer is its ability to grow and divide without any constraints. An internal clock would be a pretty big restraint. For all intents and purposes cancer has lost its internal clock. Without its internal clock, cancer can grow and divide forever—it’s now immortal. Cells in our body aren’t immortal for a reason: DNA damage. We know DNA damage happens all the time, and one way we’ve evolved to protect ourselves from DNA damage is the internal clock. Ideally, if a cell has DNA damage it won’t be passed on to great number of cells because there is a limited number of division that cell can make before the clock kicks in and stops any further divisions. This way DNA damage is contained to few cells. Without the internal clock cells with DNA damage expand uncontrollably.

telomere.jpg

Alright, so how does cancer avoid the internal clock? It stops time. The internal clock is essentially represented by the ends of chromosomes. All chromosomes are capped by a protective end of non-coding repetitive DNA, termed the telomere. Telomeres represent the internal cellular clocks. Seen in the figure, telomeres are at the ends of all chromosomes (marked in red). As previously mentioned, as we age our cells get old. Infants have ‘young’ cells and adults have ‘older’ cells. We can measure the age of cell by the length of the telomere. Infants have long telomeres and adults have shorter telomeres. As we age, our telomeres get shorter and shorter. With each division cells go through, the shorter the telomeres get. Cells with ultra short telomeres stop dividing (the Hayflick limit). This is totally normal and leads to biological ageing. Cancer cells have circumvented this problem by stabilizing their telomeres at a given length. There telomeres stop shortening and remain at a constant length. Without the shortening of telomeres (without the clock running) cancer cells can grow and divide without any limitations. Cancer is immortal.

Cancers Strength; its Greatest Downfall

How does cancer maintain a constant telomere length (stop time)? Through the reactivation of an enzyme called telomerase. Telomerase is a regular enzyme not active in most cells (with the exception of germ cells) reprogrammed to work in cancer cells. Its main function is to lengthen telomeres. It attaches to the ends of chromosomes at the telomere and elongates this protective cap. Essentially, cancer cells stop time and never get old. By elongating or maintaining the telomere size the cancer cells maintain their immortality. 90% of all human tumors have activated telomerase, confirming their immortality. Telomerase and immortality are the strength of cancer. No matter what treatments and therapies we bombard onto cancer, as long as one cell with this immortality survives the cancer will persist. Almost every cancer type utilizes this up-regulation of telomerase. From the time this was discovered, scientists and doctors have been attempting to target cancer through telomerase. Telomerase is one of the only constants between almost all types of cancers. Instead of hundreds of therapies for hundreds of types of cancers—we could be looking at one type of therapy for all types of cancers.

Future Weapons Challenging Cancer

Targeting telomerase and/or the telomere itself isn’t easy and it won’t happen tomorrow. Nevertheless, telomere/telomerase therapies might be the future and ideal targets for a global attack on cancer. Drugs that posses the ability to interfere with telomerase would revert cancer cells back into a finite replicative potential. Telomere shortening with age would resume (the cellular clock would start again) and telomere length would be lost as the cells continued to divide. Eventually, the Hayflick limit would be reached and the cancer cells would stop dividing. Several pharmaceutical companies are currently conducting clinical trials involving telomerase inhibition and telomerase vaccination. Interestly, the structural modification of the telomere seems to be a more robust and decisive cancer killer than inhibition of telomerase alone. Structurally modifing the telomere blocks the binding of telomerase to the protective cap, thereby inhibiting it’s lengthening abilities. If telomerase can’t lengthen the telomere then it can’t stop the clock. One of the most promising structural modifications to telomeres that inhibits telomerase is caused by guanine—guanine base pairing. This is a specialized form of DNA pairing called G4 DNA. G4 DNA causes a structural change in the telomere resulting in a protective cap that is no longer recognized by telomerase. Current and future cancer therapies will take advantage of G4 DNA. If we can stabilize G4 DNA in cancer cells, then telomerase will loose the ability to lengthen the telomere and cancer cells will eventually die. The frontier of cancer therapies will be to specifically target cancer cells with drugs that have little to no side affects on the surrounding tissues.

References

Canadian Cancer Society, 2008. Canadian cancer statistics. [Online] Available here, March 18, 2008.

Chromosome image. [Online image] Available here, March 18, 2008.

Blackburn EH. 1991. Structure and function of telomeres. Nature 350: 569–573.

Stewart SA and Weinberg RA (2006) Telomeres: cancer to human aging. Annual Review of Cell Developmental Biology 22:531–557

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Sandy is a graduate student in experimental medicine currently working at the BC Cancer Research Center, Terry Fox Laboratories. Outside of making a mean buffer-A solution, Sandy is an avid sport enthusiast and takes on all challengers!