DNA RESTRICTION DIGEST AND GEL ELECTROPHORESIS: A VIRTUAL LAB
This is the virtual version of the UBC Advanced Molecular Biology Laboratory’s experimental kit #2 (see Restriction Digest of Lambda DNA and Gel Electrophoresis for details) which features a common and important molecular technique used in laboratories to analyze DNA. This molecular technique can be used to isolate a gene of interest and to identify DNA sequences that are different. This molecular technique is particularly useful in forensics where it is used to analyze DNA from crime scenes! In this virtual experiment, analysis is performed on lambda DNA and will consist of two main steps. The first step is to use restriction enzymes to cut lambda DNA into fragments of different length. The second step is to perform gel electrophoresis where the DNA fragments of different length are separated by size and dyed for visualization forming a band pattern.