From June, 2006

FLOW CYTOMETRY: A TECHNOLOGY TO COUNT AND SORT CELLS

(August, 2003) Flow cytometry is a technology that allows a single cell to be measured for a variety of characteristics, determined by looking at how they flow in liquid [1]. Instruments used for this can gather information about cells by measuring visible and fluorescent light emissions, allowing cell sorting based on physical, biochemical and antigenic traits. Flow Cytometers A flow cytometer, sometimes called a Fluorescence Activated Cell Sorter (FACS), has several key components [2] (see Figure 1): 1. A light or excitation source, typically a laser that emits light at a particular wavelength; 2. A liquid flow that moves the…